Genome editing for precision crop breeding

Part 1 Genome editing techniques
1.Using TALENs for genome editing in plants: Hilal Betul Kaya, Manisa Celal Bayar University, Turkey; Rhitu Rai, ICAR-National Institute for Plant Biotechnology, India; and Adam J. Bogdanove, Cornell University, USA;
2.Double strand break (DSB) repair pathways in plants and their application in genome engineering: Natalja Beying, Carla Schmidt and Holger Puchta, Karlsruhe Institute of Technology, Germany;
3.Advances in the generation of insertion-based genome edits in plants: Baike Wang and Juan Wang, Institute of Horticulture Crops – Xinjiang Academy of Agricultural Sciences, China; Shaoyong Huang, Institute of Horticulture Crops – Xinjiang Academy of Agricultural Sciences and College of Forestry and Horticulture – Xinjiang Agricultural University, China; and Yaping Tang, Ning Li, Shengbao Yang, Tao Yang and Qinghui Yu, Institute of Horticulture Crops – Xinjiang Academy of Agricultural Sciences, China;
4.Viruses as vectors for the delivery of gene-editing reagents: Evan E. Ellison, James C. Chamness and Daniel F. Voytas, University of Minnesota, USA;
5.Progress in precise and predictable genome editing in plants with base editing: Sabine Fräbel, Shai J. Lawit, Jingyi Nie, David G. Schwark, Thomas J. Poorten and Nathaniel D. Graham, Pairwise Plants, USA;
6.Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems: Kaiyuan Chen, Hao Liu and Kabin Xie, Huazhong Agricultural University, China; Muhammad Tahir ul Qamar and Ling-Ling Chen, Huazhong Agricultural University, and Guangxi University, China;
7.Advances in assembling gRNA/Cas9 constructs in genome editing of plants: Marta Vázquez Vilar, Sara Selma, Asun Fernández del Carmen and Diego Orzáez, Instituto de Biología Molecular and Celular de Plantas de Valencia (CSIC-UPV), Spain;
8.Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants: William Gordon-Kamm, Pierluigi Barone, Sergei Svitashev, Jeffry D. Sander, Sandeep Kumar and Todd Jones, Corteva Agriscience, USA;
9.Advances in screening plants for edits and off-targets: Chun Wang and Kejian Wang, China National Rice Research Institute, China;
10.Targeted modification of promoters: Andika Gunadi and Ning Zhang, Boyce Thompson Institute, USA; and John J. Finer, The Ohio State University, USA;
11.The regulation of genome-edited crops: Gregory Jaffe, Center for Science in the Public Interest, USA;

Part 2 Applications
12.Genome editing of barley: Martin Becker, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany; and Goetz Hensel, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany, and Palacký University, Czech Republic;
13.Genome editing of maize: Jacob D. Zobrist, Morgan McCaw, Minjeong Kang, Alan L. Eggenberger, Keunsub Lee, and Kan Wang, Iowa State University, USA;
14.Genome editing of sorghum: Aixia Li, Shandong University, China; and David R. Holding, University of Nebraska-Lincoln, USA;
15.CRISPR/Cas9-mediated genome editing in Brassica: Cheng Dai, Xia Tian and Chaozhi Ma, Huazhong Agricultural University, China;
16.Genome editing of tomatoes and other Solanaceae: Joyce Van Eck, The Boyce Thompson Institute and Cornell University, USA;
17.Genome editing of woody perennial trees: Chung-Jui Tsai, University of Georgia, USA;

"CRISPR/Cas technology is revolutionizing molecular biology and has the potential to create a novel, more sustainable agriculture. With its breadth of coverage and the expertise of the distinguished international team of contributing authors, this comprehensive guide to genome editing for precision crop breeding promises to be an ideal reference for researchers and graduate students and those working in agriculture and plant biotechnology around the world."Prof. Caixia Gao, Institute of Genetics and Developmental Biology – Chinese Academy of Sciences