MALDI Mass Spectrometry Imaging

From Fundamentals to Spatial Omics

Edited by Tiffany Porta Siegel

Publication date:

23 November 2021

Length of book:

500 pages

Publisher

Royal Society of Chemistry

ISBN-13: 9781839165207

This book gathers, in a single resource, knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging. It includes fundamentals in the MALDI ionisation process, different source geometries and capabilities, detection systems and the latest research and applications in the range of –omics area as well as other broader areas. Chapters will touch on dedicated sample preparation protocols specific for the class of compounds of interest, instrumentation used with strengths and current limitations, strategies for structural analysis and identification and applications. It will be a welcomed addition to the literature in this fast-moving field and provide a guide to new innovations and applications especially in metabolomics and proteomics.

With contributions from leading experts, this book will be an authoritative guide to this method. Aimed at postgraduate and professional researchers, in academia and in the industrial market where it has direct application to clinical research. It will be a supporting volume for those just entering the field as well as experienced practitioners.

An Introduction to MALDI Ionization Mechanisms for Users of Mass Spectrometry Imaging; MALDI Mass Spectrometry Imaging - Past, Present and Future Challenges; Instrumentation for MALDI-MSI – Part I Ionization Sources and Design; Instrumentation for MALDI-MSI – Part II Detection Systems; Sample Preparation of Biological Tissues for MALDI-MSI; Quantitative MSI: A Review of Biomarker and Drug Quantifications Using Mass Spectrometry Imaging; A Glimpse into the Local Metabolic State of Cells and Tissues – Spatial Metabolomics by MALDI-MSI; MALDI-MSI in Lipidomics; MALDI Mass Spectrometry Imaging of Neuropeptides; MALDI Mass Spectrometry Imaging and Spatially-resolved Proteomics; The Role of Informatics and Data Analysis in MALDI Mass Spectrometry Imaging; MALDI-Mass Spectrometry Imaging for Microbiology; MALDI-MSI in Drug Discovery and Development; MALDI-MSI in the Forensic arena; MALDI-MSI for Toxicological Evaluation of Environmental Pollutants; MALDI-MSI in the Clinical Landscape; MALDI-MSI for Eye Diseases; Correlative Multimodal MSI – Imaging Across the Scales

'It is a very well presented, clearly written, and comprehensive book, offering a good balance between theoretical descriptions of ionisation mechanisms and instrumental setups and broad practical descriptions of application areas.'

'is a long overdue addition to the relevant literature and T. Porta Siegel et al. have made an excellent and comprehensive contribution to the MSI and MALDI MS field. I can highly recommend reading this book for prospective, new, and seasoned MSI users.'