Advanced Fragmentation Methods in Biomolecular Mass Spectrometry

Probing Primary and Higher Order Structure with Electrons, Photons and Surfaces

Edited by Frederik Lermyte

Publication date:

11 December 2020

Length of book:

342 pages

Publisher

Royal Society of Chemistry

ISBN-13: 9781839161100

BOOK DESCRIPTION
TABLE OF CONTENTS

Breaking down large biomolecules into fragments in a controlled manner is key to modern biomolecular mass spectrometry. This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods. It provides a comprehensive overview of their history, mechanisms, instrumentation, and key applications. With contributions from leading experts, this book will act as an authoritative guide to these methods.
Aimed at postgraduate and professional researchers, mainly in academia, but also in industry, it can be used as supplementary reading for advanced students on mass spectrometry or analytical (bio)chemistry courses.

Modern Mass Spectrometry and Advanced Fragmentation Methods; Mechanism and Implementation of Electron Capture and Electron Transfer Dissociation; ECD/ETD for Sequencing of Peptides and Denatured Proteins; Structural Studies Using Electron-Based Fragmentation Methods and Chemical Labelling of Proteins; Investigating 3D Structures of Native Proteins and Complexes through Electron-Based Dissociation; Electron Detachment Dissociation (EDD) and Negative Electron Transfer Dissociation (NETD); Electron Photodetachment and the Radical Fragmentation Route for Negative Ions; Elucidating Biomolecular Structure through Bond-Selective Radical Fragmentation; Structural Analysis of Lipids Using Advanced Tandem MS Methods; Photoactivation and Dissociation in Biomolecular Mass Spectrometry; Surface-induced Dissociation in Biomolecular Mass Spectrometry